Birth of a normal girl after in vitro fertilizatio...[N Engl J Med. 1992] - PubMed Result

Birth of a normal girl after in vitro fertilizatio...[N Engl J Med. 1992] - PubMed Result: "N Engl J Med. 1992 Sep 24;327(13):905-9.Links

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N Engl J Med. 1992 Sep 24;327(13):951-3.

Birth of a normal girl after in vitro fertilization and preimplantation diagnostic testing for cystic fibrosis.
Handyside AH, Lesko JG, Tarín JJ, Winston RM, Hughes MR.

Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London.

BACKGROUND. Cystic fibrosis is a common, severe autosomal recessive disease caused in a majority of cases by a three-nucleotide deletion (delta F508) in the cystic fibrosis transmembrane regulator gene. Current methods of prenatal diagnosis involve chorionic-villus sampling or amniocentesis. In vitro fertilization and diagnosis during embryonic development before implantation would allow only unaffected embryos to be selected for transfer to the uterus, thereby avoiding the need to terminate a pregnancy. METHODS. Preimplantation diagnosis of cystic fibrosis was attempted in the cases of three couples, both members of which carried the delta F508 deletion. In vitro fertilization techniques were used to recover oocytes from each woman and fertilize them with her husband's sperm. Three days after insemination, embryos in the cleavage stage underwent biopsy and removal of one or two cel"